WebInduction of UBXN2A reduces AKT phosphorylation downstream of the mTORC2 pathway, which is essential for a plethora of cellular processes, including cell migration. ... On d 5 + 3, the beating areas of the EBs were dissociated by gentle pipetting with a glass pipette with an internal diameter of 200–300 μm. Then, the cell clusters were digested into individual cells with Accutase enzyme solution (Life Technologies) for 30 min at 37 °C. The isolated cells were collected for further … See more D3 mouse ES cells (American Type Culture Collection, VA, USA) were maintained as previously described [26, 27]. Briefly, mouse ES cells (~900) were … See more Cell lysates were prepared in Western blot lysis buffer (Beyotime, Shanghai China). Mitochondrial/cytoplasmic proteins were obtained from samples with a … See more EBs obtained on d 5 + 3 of differentiation were harvested and digested into single cells with Accutase enzyme solution (Life Technologies). Cells were fixed with 4% … See more The ΔΨm of ESC-CMs was measured as previously described [26] following the manufacturer’s instructions. ESC-CMs on d 5 + 3 were plated on 48-well … See more
UBXN2A suppresses the Rictor-mTORC2 signaling pathway, an
WebOct 24, 2012 · Phosphorylation of the mTORC1 target S6 is decreased as well as the phosphorylation of the mTORC2 component Rictor on Thr1135. Finally, Akt phosphorylation on Ser473 is lost and then, cell death by necrosis occurs. Inhibition of PARP-1 with the potent PARP inhibitor AG14361 prevents all of these events. WebMar 20, 2024 · We found that Rictor phosphorylation requires mTORC1 activity and, more specifically, the p70 ribosomal S6 kinase 1 (S6K1). We identified several phosphorylation … csx right of entry
The role of RICTOR amplification in targeted therapy and …
WebFeb 22, 2011 · Immunoblotting was used to detect phosphorylation of rictor at Ser 1235, phosphorylation of Akt at Ser 473, phosphorylation of PERK at Thr 980, phosphorylation of glycogen synthase (GS) at Ser 641, phosphorylation of GSK-3β at Tyr 216 or Ser 9, and the total abundance of rictor, Akt, GS, GSK-3β, or α-tubulin in cell lysates. CTL, control. WebFeb 18, 2005 · The rictor-mTOR complex directly phosphorylated Akt/PKB on Ser 473 in vitro and facilitated Thr 308 phosphorylation by PDK1. Rictor-mTOR may serve as a drug … WebInduction of UBXN2A reduces AKT phosphorylation downstream of the mTORC2 pathway, which is essential for a plethora of cellular processes, including cell migration. ... Mechanistic studies revealed that UBXN2A targets Rictor protein, a key component of the mTORC2 complex, for 26S proteasomal degradation. A set of genetic, pharmacological, … csx rice yard