Web10 feb. 2024 · Mito-CIBN-GFP was obtained by Gibson assembly cloning, combining the mitochondrial anchor obtained from pcDNA4TO-mito-mcherry-24xGCN4_v1 (ref. 38; … WebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry标签的p62融合蛋白，呈现明亮的红色荧光，可以用于细胞自噬(autophagy)的研究

Hot Plasmids - February 2024 - Addgene

Web25 jan. 2024 · Similarly, Nb127D01-mCherry and mito-GFP-127D01 co-localized with mitochondria (Figure 1—figure supplement 2H). We next examined whether the position … WebmKate2 is a basic (constitutively fluorescent) red fluorescent protein published in 2009, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer … citation interaction eps

Detection of mitophagy in mammalian cells, mice, and yeast

WebMammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy … WebMitophagy is a cargo-specific autophagic process that recycles damaged mitochondria to promote mitochondrial turnover. PTEN-induced putative kinase 1 (PINK1) mediates the canonical mitophagic pathway. However, the role of PINK1 in diseases where mitophagy has been purported to play a role, such as colorectal cancer, in unclear.Our results here … WebSimilar to the autophagic flux reporter, mito-QC is a tandem mCherry–GFP probe targeted to the outer mitochondrial membrane (OMM) that labels mitochondria red-green. When the organelle is delivered to the lysosomes, the acidic environment of the lysosome quenches the GFP fluorescence, whereas the mCherry signal remains stable. citation in research methodology