WebApr 5, 2024 · The medium was supplemented with one of these three types of cytokinin (CK): BA, meta-topolin (m-T), or thidiazuron (TDZ, Duchefa Biochemie, Haarlem, The Netherlands), all of them at 8.8 µΜ. The explants were placed in the growth chamber at the same conditions described above (Section 2.3.1. Experiment 1). WebThe root tips were digested in a pectolytic enzyme mixture [0.1% (w/v) pectolyase Y23 (Duchefa, Haarlem, the Netherlands), 0.1% (w/v) cellulase onozuka RS (Duchefa, Haarlem, the Netherlands) and 0.1% (w/v) cytohelicase (Sigma-Aldrich, Steinheim, Germany)] in 10 mM citrate buffer (10mM tri sodium citrate 10 mM citrate, pH 4.5) at 37 …
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WebMS vitamins, 30 g1-l sucrose, and 0.49 pM IBA (Duchefa, Haarlem, The Netherlands) without any gelling agents. It was sterilized under the same conditions as the bagasse. The pH of the medium was adjusted to 5.8 with 1.0 M KOH before autoclaving. For each culture flask (200 ml volume), WebMar 27, 2024 · All chemicals used in culture were purchased from Duchefa Biochemie (Haarlem, The Netherlands). 4.2. Tuber Induction. Single-node cuttings were conducted following a previous report . Plantlets were grown in culture tubes under long-day conditions, and then transferred to SD conditions for 6 days. Plants were then cut to one-node stem … community physio doncaster
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WebMar 17, 2014 · Seeds of tomato were sown on half-strength Murashige and Skoog medium with 2 g/L Gelrite Gellan Gum (Sigma-Aldrich, Milan, Italy) in covered plastic sterile vessels (Duchefa Biochemie, Haarlem, The Netherlands), and then plants were grown in the dark for 5 days at 24 °C. WebApr 11, 2024 · Sterilized stem domes with basal parts (2 mm diameter and 3 mm length) were excised from cloves and bulbils. For the osmotic dehydration, explants were placed in 9 cm Petri dishes on a Murashige and Skoog (MS) modified medium (Duchefa Biochemie, Haarlem, The Netherlands) supplemented with 13.7% sucrose and 0.8% plant agar. … WebOct 25, 2016 · Biotinylated proteins are visualized by staining with 5-bromo-4-chloro-3-indoyl phosphate (BCIP) and nitroblue tetrazolium (NBT) (Duchefa, Haarlem, The Netherlands) in 0.1 M Tris–HCl, pH 9.5, 5 mM MgCl 2, 0.15 mg/mL BCIP, 0.30 mg/mL NBT. To avoid background staining, we recommend to wash the membranes (twice 10 min) with TBST … community physio services