2024 Clontech pgbkt7

Clontech pgbkt7

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August undefined, 2024

WebFeb 19, 2013 · The BIK1 coding region was cloned into pGBKT7 (Clontech) and introduced into yeast cells carrying an Arabidopsis cDNA library via a mating procedure (Clontech). Approximately 2 × 10 7 yeast clones were screened with BIK1 as bait. To verify interactions between the PEPR1 C terminus (amino acids 1025–1123) and various BIK1 family … WebMap of pGBKT7-53 DNA-BD Control Vector. 55 Figure 19. Map of pGBKT7-Lam DNA-BD Control Vector. 56 Notice to Purchaser Clontech products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited to, …

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WebThe VP37 bait vector (pGBKT7-37) was constructed using the appropriate restriction enzyme (TaKaRa Bio Inc., Otsu, Japan), in order to clone the VP37 genes into pGBKT7 (Clontech Laboratories). WebApr 21, 2024 · Saccharomyces cerevisiae strain AH109, human fetal brain cDNA library that was constructed on pACT2 fused with the GAL4 activation domain, and control vectors pGADT7, pGADT7-T, pGBKT7-p53, and pGBKT7-Lam were from Clontech. pGBKT7-UL44 was first introduced into strain AH109 and verified that do not activate the report … post register classifieds jobs

Screening and identification of host proteins interacting with

WebGAL4 DNA-binding domain (DNA-BD) in pGBKT7 vector (Clontech) with the in-fusion cloning method (Clontech). The vector pGBKT7 vector was linearized by primers, infusionA9 and infusionB9. Seven ripG genes, ripG1, ripG2, ripG3, ripG4, ripG5, ripG6, and ripG, were amplified from OE1-1 genome DNA by pairs of primers (Table 2), … WebDec 5, 2008 · pGBKT7 Vector Information. Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3248-5 2 Version No. PR8Y2643. Location of features: • Truncated S. cerevisiae ADH1 . promoter (P. ADH1): 30–736 • GAL4 DNA binding domain (DNA-BD) … WebAbout our brands: Clontech. Our Clontech brand represents over 30 years of service to the life sciences research community through the development, commercialization, and support of kits and reagents for diverse experimental applications, with emphases in cell … total size of automobile industry

BIK1 interacts with PEPRs to mediate ethylene-induced immunity

Envelope Proteins of White Spot Syndrome Virus (WSSV) Interact …

WebEmpty pGBKT7 encoding the GAL4 DBD served as a negative control. Article Snippet: Full-length HvNAC005 and a series of C-terminal deletion derivatives, with or without the NAC domain, were cloned into the DBD vector pGBKT7 ( Clontech ) using primers listed in Supplementary Table S1 . WebHow and where to buy legal weed in New York – Leafly. How and where to buy legal weed in New York. Posted: Sun, 25 Dec 2024 01:36:59 GMT [] post register in idaho falls idaho obituariesWebOct 19, 2024 · pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. post register texas falls obituary

"WebApr 3, 2007 · Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3247-1 Version No. PR742219 I. Introduction continued Optimized vectors facilitate downstream confirmation The Matchmaker System 3 DNA-BD and AD fusion vectors, pGBKT7 and pGADT7, were designed for high-level protein expression and to facilitate confirmationof … " - Clontech pgbkt7

Clontech pgbkt7

Sucrose Signaling Regulates Anthocyanin Biosynthesis Through …

WebAug 21, 2013 · pGBKT7 Vector Information. Use: pGBKT7 is the DNA-BD Vector included with Clontech's Matchmaker Systems. The MCS of pGBKT7. contains unique restriction sites in frame with the 3' end of the GAL4 DNA-BD for constructing fusion proteins. with a bait protein. The bait protein is also expressed as a fusion to a c-Myc epitope tag. c-Myc … WebOct 15, 2007 · pGBKT7Prey vector, GAL4 41–147 DNA-BD, LEU2, Km R, c-Myc epitope tag: Clontech pGBKT7-BETF-β-BD fusion in pGBKT7; Km R: This study pGBKT7-PFMPFM-BD fusion in pGBKT7; Km R: This study pGADT7-TSV40 large T-antigen in pGADT7, LEU2, Ap R: Clontech pGBKT7-53Murine p53 in pGBKT7, TRP1, km R: …

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WebJun 23, 2016 · Plasmids. Prior to performing the yeast two-hybrid assay, we constructed the bait plasmid. The full-length coding sequence of TSC22D2 was amplified by PCR and inserted into pGBKT7 (Clontech, Mountain … WebControl plasmids pGBKT7-53, pGADT7-T, and pGBKT7-Lam were used as positive and negative controls as per manufacturer’s instructions (Clontech). Techniques: Generated, Growth Assay Journal: eLife

WebAug 16, 2024 · The Y2H analysis was carried out using a GAL4-based Y2H system from MatchmakerGold Systems (Clontech, Palo Alto, CA). The full-length complementary DNA (cDNA) of YDA was cut with NotI and SfiI and ligated into pGBKT7 to construct a bait plasmid. ... (Clontech). pGBKT7-Lam and pGADT7-T were used as negative controls. … WebApr 11, 2024 · The yeast two-hybrid experiments were performed according to the Yeast Handbook (Clontech, USA). Briefly, full length cDNA of OsUGE1 was amplified and inserted into pGADT7 vector and pGBKT7 vector, respectively. Then fused vectors were co-transformed into yeast strain AH109 and incubated for 3 days at 30 ℃ on medium …

WebPgbkt7 Lam Control Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results ... (Clontech by Takara Bio, Mountain View, ... WebThe Nco I and Pst I sites may be used to shuttle inserts from pGADT7 into pGBKT7, the Matchmaker Two-Hybrid System 3 DNA-BD Vector.The MCS in pGADT7 is compatible with those in pMyc-CMV and pHA-CMV, Clontech's epitope tagged mammalian expression …

Web1 day ago · To construct AD/BD recombinant vectors, the ORFs of four Aux/IAA genes (DoIAA1, 6, 10 and 13) were cloned into the pGBKT7 vector (Clontech, Palo Alto, CA, USA), and the ORFs of three ARF genes (DoARF2, 17 and 23) were cloned into the pGADT7 vector (Clontech). Two vectors (containing DoIAAs-BD and DoARFs-AD) were …

WebpGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast … post register readers choice awardsWebPlasmid pGBKT7-GW from Dr. Yuhai Cui's lab is published in Plant J. … post register readers choice awards 2021 post register subscribers servicesWebpGBKT7 Vector Information CLONTECH Laboratories, Inc. INTERNET: www.clontech.com Protocol # PT3248-5 2 Version # PR8Z150 Location of features: • Truncated S. cerevisiae ADH1 promoter (P ADH1): 30–736 • GAL4 DNA binding domain (DNA-BD) polypeptide … post register vacation holdWebDescription from Clontech: "pM is used to generate a fusion of the GAL4 DNA-BD (amino acids 1–147) and a protein of interest in the Matchmaker™ Mammalian Assay Kit 2 (Cat. No. 630305). The hybrid protein is targeted to the cell’s nucleus by the GAL4 nuclear … total size of indian budget 2022-23WebShow Static Map. Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. Efficient cleavage requires at least two copies of the NaeI recognition sequence. Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends ... total size of forza horizon 5WebArticle Snippet: AH109 cotransformed with murine pGBKT7-p53/pGADT7-T (supplied by Clontech) was used as positive control, whereas yeast cotransformed with pGADT7/pGBKT7 were used as negative control. β-galactosidase assay was performed as per the manufacturer's protocol. Techniques: Transformation Assay, Construct total size of gameloop